lepr cre (Jackson Laboratory)
86
Structured Review
Jackson Laboratory
lepr cre
Lepr Cre, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lepr cre/product/Jackson Laboratory
Average 86 stars, based on 1 article reviews
Lepr Cre, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lepr cre/product/Jackson Laboratory
Average 86 stars, based on 1 article reviews
lepr cre - by Bioz Stars,
2026-06
86/100 stars
Images
1) Product Images from "GREM1 acts in leptin receptor-expressing skeletal cells to mediate peri-implant fibrosis"
Article Title: GREM1 acts in leptin receptor-expressing skeletal cells to mediate peri-implant fibrosis
Journal: Nature Communications
doi: 10.1038/s41467-026-70111-0
Figure Legend Snippet: a Conditional knockout strategy for Grem1 in LEPR-lineage cells (LepR cre/+ ; Grem1 f/f ) and the corresponding cre-only littermate control (LepR cre/+ ). Created in BioRender.,v.(2025) https://BioRender.com/i4syixo . b μCT reconstructions of proximal tibiae from LepR cre/+ and LepR cre/+ ; Grem1 f/f mice at postoperative day 14 after fibrous-integration surgery. Scale bar, 500 µm. Yellow rasterized area indicates implant; red rastered area indicates fibrotic area. BV/TV ( c ) and trabecular thickness ( d ) (LepR cre/+ n = 13; LepR cre/+ ; Grem1 f/f n = 14; unpaired, two-tailed Student’s t test; mean ± s.d.). e H&E staining of proximal tibiae at day 14 (scale bar, 500 µm). Histologic quantification of peri-implant fibrosis ( f ) and peri-implant bone ( g ) at day 14 (LepR cre/+ n = 13; LepR cre/+ ; Grem1 f/f n = 14; unpaired, two-tailed Student’s t test; mean ± s.d.). h, Flow cytometric quantification of Lin− LepR-tdTomato+ cells at day 14 ( n = 5 for LepR cre/+ and LepR cre/+ ; Grem1 f/f ; unpaired, two-tailed Student’s t test; mean ± s.d.). i Representative immunofluorescence showing LepR-tdTomato (red) and CD200 (green) in peri-implant fibrotic tissue. Scale bar, 500 µm. The middle row shows magnified views of the boxed regions (LepR cre/+ (red box); LepR cre/+ ; Grem1 f/f (blue box)). Scale bar, 50 µm. Quantification from immunofluorescence of peri-implant LepR-tdTomato+ cells ( j ) and CD200+ LepR-tdTomato+ cells ( k ) at day 14 ( n = 6 for LepR cre/+ and Lepr cre/+ ; Grem1 f/f ; unpaired, two-tailed Student’s t test; mean ± s.d.). l, Flow cytometric quantification of Lin - LepR-tdTomato + CD200 + cells at day 14 ( n = 5 for LepR cre/+ and LepR cre/+ ; Grem1 f/f ; unpaired, two-tailed Student’s t test; mean ± s.d.). Pullout testing: failure load ( m ) and work to failure ( n ) at day 14 (LepR cre/+ n = 22; LepR cre/+ ; Grem1 f/f n = 20; unpaired, two-tailed Student’s t test; mean ± s.d.). Each dot in ( c , d , f , g , h , and j – n ) represents a biologically independent replicate. Images in ( b , e , and i ) are representative of at least three independent biological replicates. Source data are provided as a Source Data file.
Techniques Used: Knock-Out, Control, Two Tailed Test, Staining, Immunofluorescence
Figure Legend Snippet: In vivo BrdU incorporation to assess proliferation of LepR-tdTomato+ cells in the peri-implant region. Representative immunofluorescence images are shown for LepR cre ;Rosa26 tdtomato ; Grem1 +/+ (Lepr cre/+ , a , b ) and LepR cre ;Rosa26 tdtomato ; Grem1 f/f (Lepr cre/+ ; Grem1 f/f , c , d ). Scale bar, 500 µm. Panels b and d are magnified views of the yellow boxed regions in ( a and c ), respectively (scale bar, 50 µm). μCT 3D reconstructions of bone nodules generated from transplanted Lin - LepR-tdTomato + cells derived from from LepR cre ;Rosa26 tdtomato ; Grem1 +/+ (Lepr cre/+ ; e ) and LepR cre ;Rosa26 tdtomato ; Grem1 f/f (Lepr cre/+ Grem1 f/f ; j ) (scale bar, 500 µm). f Representative immunofluorescence of a bone nodule derived Lin - LepR-tdTomato + cells from Lepr cre/+ . (scale bar, 50 µm). Representative Movat pentachrome ( g ), von Kossa ( h ), and Safranin O–Fast Green ( i ) staining of nodules derived from Lin - LepR-tdTomato + cells from Lepr cre/+ . (scale bars, 50 µm). k Representative immunofluorescence of a nodule derived Lin - LepR-tdTomato + cells from Lepr cre/+ Grem1 f/ (scale bar, 50 µm). Representative Movat pentachrome ( l ), von Kossa ( m ), and Safranin O–Fast Green ( n ) staining of nodules derived Lin - LepR-tdTomato + cells from Lepr cre/+ Grem1 f/ (scale bars, 50 µm). o Quantification of BrdU + LepR-tdTomato + cells in the peri-implant region ( N = 5 per group; mean ± s.d.; two-tailed unpaired t test). p, μCT quantification of bone volume of nodules derived from Lin− LepR-tdTomato + cells from LepR cre/+ and LepR cre ; Grem1 f/f ( N = 6 per group; mean ± s.d.; two-tailed unpaired t test). Each dot in o and p denotes a biologically independent replicate. Images in ( a – n ) are representative of at least three independent experiments. Source data are provided as a Source Data file.
Techniques Used: In Vivo, BrdU Incorporation Assay, Immunofluorescence, Generated, Derivative Assay, Staining, Two Tailed Test
Figure Legend Snippet: a Principal component analysis (PCA) of RNA-seq performed after FACS isolation of Lepr-tdTomato + populations from Lepr cre/+ (cont) and Lepr cre/+ Grem1 f/f (flox) mice subjected to fibrous-integration surgery and harvested at postoperative day 14. b BMP pathway genes are significantly enriched in Lin − LepR-tdTomato + cells isolated from Lepr cre/+ ; Grem1 f/f mice compared with Lin − LepR-tdTomato + cells from Lepr cre/+ controls. c WNT pathway genes are significantly enriched in Lin − LepR-tdTomato + cells isolated from Lepr cre/+ ; Grem1 f/f mice compared with Lin − LepR-tdTomato + cells from Lepr cre/+ controls. LepR-tdTomato with pSMAD staining in peri-implant fibrotic tissue at day 14 for LepR cre/+ ; Grem1 f/f ( d ) and LepR cre/+ ( e ). Scale bar, 500 µm. The right columns show magnified views of the yellow boxed regions in d and e (scale bar, 50 µm). LepR-tdTomato with active β-catenin staining in peri-implant fibrotic tissue at day 14 for LepR cre/+ ; Grem1 f/f ( f ) and LepR cre/+ ( g ). Scale bar, 500 µm. The right columns show magnified views of the yellow boxed regions in ( f and g ) (scale bar, 50 µm). Images in ( d – g ) are representative of at least three independent experiments. h Quantification of pSMAD + LepR-tdTomato + cells in LepR cre/+ ; Grem1 f/f versus LepR cre/+ mice at day 14 ( n = 6 per group; unpaired, two-tailed Student’s t test; mean ± s.d.). i Quantification of active β-catenin + LepR-tdTomato + cells in LepR cre/+ ; Grem1 f/f versus LepR cre/+ mice at day 14 ( n = 6 per group; unpaired, two-tailed Student’s t test; mean ± s.d.). Source data are provided as a Source Data file.
Techniques Used: RNA Sequencing, Isolation, Staining, Two Tailed Test
Figure Legend Snippet: a Study design for intra-articular anti-GREM1 administration to prevent peri-implant fibrosis. All LepR cre ;Rosa26 tdtomato mice underwent fibrous-integration surgery and were then randomized to receive intra-articular anti-GREM1 or isotype control. Created in BioRender,v(2025) https://BioRender.com/i4syixo . b μCT of proximal tibiae at postoperative day 14 from mice treated with anti-GREM1 or isotype. Scale bar, 500 µm. Yellow rastered area indicates implant; red rastered area indicates fibrotic area. BV/TV ( c ) and trabecular thickness ( d ) (anti-GREM1 n = 4; isotype n = 4; unpaired, two-tailed Student’s t test; mean ± s.d.). e H&E staining of proximal tibiae at day 14 (scale bar, 500 µm). Histologic quantification of peri-implant bone ( f ) and peri-implant fibrosis ( g ) (anti-GREM1 n = 4; isotype n = 4; unpaired, two-tailed Student’s t test; mean ± s.d.). h Flow cytometric quantification of Lin− LepR-tdTomato+ cells at day 14 (isotype n = 4; anti-GREM1 n = 4; unpaired, two-tailed Student’s t test; mean ± s.d.). i Representative immunofluorescence images from isotype-treated (left) and anti-GREM1-treated (right) proximal tibiae. Scale bar, 500 µm. The middle row shows magnified views of boxed regions (isotype red box; anti-GREM1 blue box) (scale bar, 50 µm). Quantification from immunofluorescence at day 14 of peri-implant LepR-tdTomato + cells ( j ) and CD200 + LepR-tdTomato + cells ( k ) (isotype n = 4; anti-GREM1 n = 4; unpaired, two-tailed Student’s t test; mean ± s.d.). l Flow cytometric quantification of Lin - LepR-tdTomato + CD200 + cells at postoperative day 1 (isotype n = 4; anti-GREM1 n = 4; unpaired, two-tailed Student’s t test; mean ± s.d.). Pullout testing at postoperative day 14: failure load ( m ) and work to failure ( n ) (isotype n = 10; anti-GREM1 n = 10; unpaired, two-tailed Student’s t test; mean ± s.d.). Each dot in ( c , d , f , g , h , and j – n ) represents a biologically independent replicate. Images in ( b , e , and i ) are representative of at least three independent biological replicates. Source data are provided as a Source Data file.
Techniques Used: Control, Two Tailed Test, Staining, Immunofluorescence
Figure Legend Snippet: a Treatment paradigm for reversal of peri-implant fibrosis via intra-articular anti-GREM1. All LepR cre ;Rosa26 tdtomato mice underwent fibrous-integration surgery; at postoperative day 14, mice were randomized to receive intra-articular anti-GREM1 or isotype for 14 days.Created in BioRender.,v.(2025) https://BioRender.com/i4syixo . b μCT of proximal tibiae from mice treated with anti-GREM1 or isotype. Scale bar, 500 µm. Yellow rastered area indicates implant; red rastered area indicates fibrotic area. BV/TV ( c ), trabecular number ( d ), and trabecular spacing ( f ) (anti-GREM1 n = 5; isotype n = 4; unpaired, two-tailed Student’s t test; mean ± s.d.). e H&E staining of proximal tibiae from anti-GREM1 versus isotype groups (scale bar, 500 µm). Histologic quantification of peri-implant fibrosis ( g ) and peri-implant bone ( h ) (anti-GREM1 n = 5; isotype n = 4; unpaired, two-tailed Student’s t test; mean ± s.d.). i Flow cytometric quantification of Lin - LepR-tdTomato + cells (isotype n = 4; anti-GREM1 n = 5; unpaired, two-tailed Student’s t test; mean ± s.d.). j Representative immunofluorescence images of proximal tibiae from isotype (left) and anti-GREM1 (right) groups. Scale bar, 500 µm. Middle row shows magnified views of boxed regions (isotype red box; anti-GREM1 blue box) (scale bar, 50 µm). Quantification from immunofluorescence of peri-implant LepR-tdTomato + cells ( k ) and CD200 + LepR-tdTomato + cells ( l ) (isotype n = 4; anti-GREM1 n = 5; unpaired, two-tailed Student’s t test; mean ± s.d.). m Flow cytometric quantification of Lin− LepR-tdTomato+CD200+ cells (isotype n = 4; anti-GREM1 n = 5; unpaired, two-tailed Student’s t test; mean ± s.d.). Pullout testing: failure load ( n ) and work to failure ( o ) (isotype n = 9; anti-GREM1 n = 10; unpaired, two-tailed Student’s t test; mean ± s.d.). Each dot in ( c – d , f – g , h – i , and k – o ) corresponds to a biologically independent replicate. Images in ( b , e , and j ) are representative of at least three independent biological replicates. Source data are provided as a Source Data file.
Techniques Used: Two Tailed Test, Staining, Immunofluorescence
